Microbiology lab report on Escherichia coli and Staphylococcus aureus.

Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this lab experiment devil pivotal types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on quad variant types of nutrient agar in order to lionise the vary morphologies deep down the colonies. Resulting data was analyzed to stick out understanding of the using up of differing culture media and conditions for bacterial ontogenesis. RESULTSFour different agar types were use in this experiment. The first ( food for thought) brooked for proceeds of two E. coli and S. aureus. The second agar used (MKL) inhibited the egression of S. aureus besides allowed the growth of E. coli. The third (ash tree) way outed growth of both bacterial species. The quarter (mannitol) was found to inhibit growth of E. coli (Table 1). Table 1. sign of growth within the four types of agar. G = growth, NG = no growth. E. coliS. aureusMixed cultureNutri entGGGMKLGNGGASHGGGMannitolNGGGChanges in colour within the sampled cultures were similarly noted. No transfers occurred within the Nutrient agar. The MKL agar exhibited a color change with E. coli and the mixed culture. The agar changed from red to purple, and a evident border was present surround the colonies that exhibited growth on the dish. The ASH agar did not yield a color change. The Mannitol agar exhibited a change with S. aureus and the mixed culture, but no change with E. coli (Table 2). Table 2. Indication of color change within the four types of agar. E. coliS. aureusMixed cultureNutrientTransparentTransparentTransparentMKLRed to PurpleRedRed to PurpleASHRedRedRedMannitolRedRed to YellowRed to YellowCONCLUSIONS AND DISCUSSIONTwo noticeable changes occurred during this experiment. First, two of the twelve inoculated Petri dishes yielded no growth. In order to explain these occurrences, we moldiness first examine the differing properties of the types of agar us ed. Nutrient agar is enriched to allow grow! th of a coarse variety... If you want to get a full essay, order it on our website: OrderCustomPaper.com

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